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Describe how you would prepare 1.000 L of 0.05M HEPES buffer @ pH 7.45 in the lab. You have pure HEPES, a pH meter, 1.00 M HCl and 1.00 M NaOH, and all of the pure water and glassware you need. Carefully go through all the mathematical steps, and then describe how you would make the buffer in the laboratory.
If one added 0.01 M of CaCl2 to this solution, how many moles of Ca(OH)2 will now dissolve in 1.0 L of water containing this concentration of CaCl2
What potential issues might arise if the pH of your buffer is too close to the pI of your protein of interest? Why might an extreme buffer pH ( 9) be unsuitable?
suppose that there is something in your sample that is absorbing at the same wavelength as the material you are looking
How many moles of chloroform, CHCl3, are required to fill a 253-mL flask at 100.0°C and 940 torr?
To keep ionic strength constant and maintain a more consistent matrix in a solution where ions could be influencing each other, another component is often added to the solution
calculate the energy difference between the spin up and spin down levels of a hydrogen atom in the 1 state when it
Data for the decomposition of hydrogen peroxide at some set temperature T is include below. The rate law depends only on the concentration of H2O2.
If the truck driver slams on the brakes, the kinetic energy of the truck is changed to heat as the brakes slow the truck to a stop. how much would the temperature of 6.91 gallons of water increase if all this heat could be absorbed by the water
a water soluble salt forms a precipitant during Group 3 analysis. An acidic solution of this precipitant and NaBiO3 yields a pinkish color. This same salt has no reaction to concentrated H2SO4 or AgNO3, what is the chemical formula for this salt?
If an aqueous solution has a hydroxide ion concentration ([OH-]) of 5.94 x 10-5 mol/L, what is the hydronium ion concentration? 4.48 x 10-12 mol/L 1.68 x 10-10 mol
Calculate the cell emf for the following reaction: 2Ag+(0.30 M) + H2(1 atm) 2Ag(s) + 2H+(pH = 7.0) Need step by step instructions
Draw resonance structures for the carbocation that could be formed from (E)-2-butenyl trifluoromethanesulfonate. (2) One of the resonance structures for this carbocation should be a more important contributor to the resonance hybrid than the other
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