Explain how you would prepare of hepes buffer

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Reference no: EM13651237

Describe how you would prepare 1.000 L of 0.05M HEPES buffer @ pH 7.45 in the lab. You have pure HEPES, a pH meter, 1.00 M HCl and 1.00 M NaOH, and all of the pure water and glassware you need. Carefully go through all the mathematical steps, and then describe how you would make the buffer in the laboratory.

Reference no: EM13651237

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