Explain how would adjust the concentration of tween

Assignment Help Chemistry

Reference no: EM13184620

Tween-20 is a detergent that is often used in immunological assays. Concentrated Tween-20 a viscous liquid and is difficult to accurately pipette in small amounts. Because of thism it is common to make a 10% stock of Tween 20 i nwater and use this to add to other solutions such as PBS. It is generally used at fairly low concentrations in the range of .1-1%. PBS containing 0.1% Tween-20 (PBST) is commonly used as a wash solution in ELISA. Someone in your lab has made an error in making upthis .1% solution. They inadvertently prepared 400mL containing 0.04% Tween 20. How would you adjust the concentration of Tween 20 in this stoct to 0.1% usng a 10% Tween 20 Stock? Hint: First determine how much 10% Tween 20 was used to make a .04% soluntion/\; then how much should have been used to make a 0.1% solution. What is the difference in volumes?

Reference no: EM13184620

Questions Cloud

State what is the overall charge state for the tetrapeptide : 1) What is the overall charge state for the tetrapeptide DREAM at physiological pH. The pK values for N-terminal, C-terminal, side chain of asp, arg, glu are 9.0, 3.5, 3.9, 12.5, and 4.1

Compute the stoichiometric air-fuel ratio : calculate the stoichiometric air-fuel ratio of a fuel blend containing 80 vol.% ethanol and 20 vol.% gasoline

Why the company thought they would be flatter or steeper : Why does google care weather people think it is large or small? Do highway billboards actually provide competition for Google. Briefly explain. Please state wheather the actual demand were a)to the right or to the left the where the company thou..

State aluminum was reacted with hydrochloric acid : a 1.00 g sample of an alloy of copper and aluminum was reacted with hydrochloric acid , and 85.0 mL of hydrogen gas (measured atSTP) was produced.

Explain how would adjust the concentration of tween : Tween-20 is a detergent that is often used in immunological assays. Concentrated Tween-20 a viscous liquid and is difficult to accurately pipette in small amounts. Because of thism it is common to make a 10% stock of Tween 20 i nwater and use this..

Balance sheet as the par value of common stock : In mid- 2014 due to dramatic increases in profits, the stock reached a market value of $90 per share. The board of directors approved a 2- for -1 split. After the stock split, what will the balance sheet show as the par value of common stock?

Identify the comparative advantage enjoyed by each country : Further, assume that the countries have similar resource endowments and that, initially, they are not trading with each other. Therefore, each of the countries has to produce both rice and tea for its citizens. Suppose that, in the no-trade situat..

Determine the balance sheet inventory carrying value : Determine the balance sheet inventory carrying value at December 31,2011 assuming the LCM rule is applied to individual products and separate income statement item, determine the amount of the loss.

State the thermocouple is then placed in boiling toluene : The thermocouple is then placed in boiling toluene at the same pressure and provides an output of 12.59 mA.

User Account

All Pages