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Explain how will the net charge of a typical protein change

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The table below indicates approximate values for these at 25C. For reference, the delta H(ionization) for Tris is 47kJ/mole.

Group deta H (ionization)(kJ/mole)
Asp 3.9
Glu 0
Lys 48
Arg 48
His 28
N-term 40
C- term 0

How will the net charge of a typical protein change upon lowering the temperature from 37C to 4C, based on this information?

What can be done experimentally to compensate for this?

Reference no: EM1339053

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