Explain how to separate pyridine pka of conjugate acid

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your lab manager has asked your lab partner to separate Pyridine Pka of conjugate acid:5.30 and phenol pka :10 using reverse phase HPLC, you and your lab partner are both eligible for the same promotion, and while you have have nothing against your lab partner, you really want the promotion. you decided to sabotage your lab partner;s to separate the two compounds . list two ways that you could adjust your lab mobile phase that would almost guarantee a more difficult separation. explane your reasoning?

Reference no: EM13518724

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