Explain how to purify a certain protein

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In order to purify a certain protein, you require 0.1 M glycine buffer at pH 9.4. Unfortunately, your stockroom has run out of glycine. However, you manage to find two 0.1M glycine buffer solutions. one at pH 9.0 and the other at pH 10.0. What volumes of these two solutions must you mix in order to obtain 200 mL of your required buffer

Reference no: EM13266431

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