Explain hcl would you require to make 1 l of the buffer

Reference no: EM13558619

For an enzyme assay you require a 50 mM Tris-HCl buffer, pH 7.8. How many grams of Tris (mw = 121, pKA = 8.1) and how any ml of 2 M HCl would you require to make 1 L of this buffer?

Reference no: EM13558619

Questions Cloud

Explain what volume of .01m hcl added to phosphate buffer : What volume of .01M HCL must be added to 20 ml of .04M phosphate buffer, pH 6.50, to reduce the pH to 5.5. pK of phosphate buffer is 7.20

Define what is the concentration of the final solution : After the second dilution 100.00ml of that solution where transferred to a 150.0 mL flask and it was diluted to the mark. What is the concentration of the final solution

Find the electrostatic force acting on each particle : In the bohr theory of the hydrogen atom an electron moves in acircular orbit about a proton. the radius of the orbit is0.53x10^-10m. find the electrostatic force acting on each particle

An extreme amusement park ride accelerates : An extreme amusement park ride accelerates its riders upward from rest to 50.0 m/s in 7.00 seconds. Ignoring air resistance, what average upward force does the seat exert on a rider who weighs 1120 N?

Explain hcl would you require to make 1 l of the buffer : For an enzyme assay you require a 50 mM Tris-HCl buffer, pH 7.8. How many grams of Tris (mw = 121, pKA = 8.1) and how any ml of 2 M HCl would you require to make 1 L of this buffer

Evaluate what is the total resistive force : At a certain part of yourdrive from home to school, your car (mass = 1400 kg) will coast inneutral at a constant speed of 84 km/h if there is no wind. What is the total resistive force

Calculate volumetric flow rate and mass flow rate : Calculate the volumetric flow rate and the mass flow rate(ENMIN 5023 Mine Ventilation)- determine the average flow velocity of the air and the air quantity flowing - what particular precautions have to be taken when undertaking such a traverse?

What is the index of refraction of the plate of glass : The problem is determining the index of refraction. A microscope focuses on a tiny scratch in the upper surface of a plate of glass,then the barrel position is recorded. Next the microscope is lowered by 1.87mm and a focused image of the scratch i..

Explain what is the ph of the na2hpo4 buffer : A buffer is prepared by mixing 500 ml of 0.4 M Na2HPO4 with 500 ml of 0.2 M NaH2PO4. what is the pH of this buffer? What would be the final pH if you added the same quantity of acid to 1 litre of H2O (pH 7)

User Account

All Pages