Explain geometry of the chromatographic column

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Reference no: EM13188329

1) To characterize the elution properties of the solute in a way independent of the geometry of the chromatographic column, the retention constant R = Vo / Ve is used. R is equal to the fraction of solute in the void volume Vo and (1 - R) is the fraction of solute in the pore volume Vp during the chromatographic run. Show that (1 - R) / R = kÃ¢ï¿½ï¿½ [NOTE: k' = the column capacity factor (Ve - Vo) / Vo]

2) Given the E1% = 21.9 for cytochrome c, how much potassium ferricyanide is required to react with 10 mL of a cytochrome c solution with an absorbance of 2.0 for a 1 cm path length? NOTE: E1% = 21.9 means that a 1% solution of cytochrome-c has an absorbance of 21.9. Potassium ferricyanide = K3Fe(CN)6. Molecular weight of cytochrome-c = 12,200 g/mol

Reference no: EM13188329

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