Explain fluorescence resonance energy transfer

Reference no: EM13333196

Fluorescence resonance energy transfer (FRET) is a common technique to measure distances between donor (D) and acceptor (A) fluorescent molecules. In the absence of A, the fluorescence lifetime of a protein labeled with D is 23 ns. In the presence of the A label on the protein, the lifetime falls to 18 ns. What is the distance between these two labels if the r0 for the pair is 34 Å?

Reference no: EM13333196

Questions Cloud

Develop a draft proposal for consideration by the board : Develop a draft proposal for consideration by the Board and Management of each company. In your proposal you should indicate:

Compute the magnitude of the net force acting on this object : A 3.25-kg object is moving in a plane, with its x and y coordinates given by x = 2t2 ? 4 and y = 4t3 + 4, Find the magnitude of the net force acting on this object

Explain phenomena generate hypotheses : Theory can offer many things to the researcher. It can be used to explain phenomena, generate hypotheses and suggest additional directions for research. In turn, the results from research can be used to refine existing theory.

Calculate the net expected value for the project risks : Calculate the net expected value for the project risks and opportunities cited above. How much should you plan for your contingency reserve budget based on the above? You must show all of your calculations.

Explain fluorescence resonance energy transfer : Fluorescence resonance energy transfer (FRET) is a common technique to measure distances between donor (D) and acceptor (A) fluorescent molecules

Explain purine and pyrimidine bases in dna results : The to * transitions of purine and pyrimidine bases in DNA results in absorbance of light at 260 nm. If the extinction coefficient for a PCR primer of DNA is 1.00 x 104 M-1 cm-1

Find the direction of this the magnetic force : A group of particles is traveling in a magnetic field of unknown magnitude and direction. What is the direction of this the magnetic force

Explain extraction of an aqueous solution : suppose that you are performing an extraction of an aqueous solution with an organic solvent that has a density less than water. Propose a method to identify and collect only the organic solvent. Assume that water and your solvent of interest are ..

Sheet metal roofing on large warehouse structure : Smith Roofing Company installs sheet metal roofing on large warehouse structures. This is done by placing a bundle of sheet metal "decking" on the roof, and then taking the top sheet of the bundle and putting it in place while standing on the bundle.

User Account

All Pages