Explain cleave with trypsin which cleaves on the cooh

Reference no: EM13533164

You have an purified small protein the you're trying to sequence. You cleave a sample of your small protein with CNBr which cleaves on the COOH side of M (methionine) and you get 2 peptides. With a separate sample of your small protein you cleave with trypsin which cleaves on the COOH side of K (lysine) and R (arginine) and you get 3 peptides. The 5 peptides have the following amino acid sequences: Peptide 1: VALKTQAM Peptide 2: LDGKM Peptide 3: TQAM Peptide 4: LDGK Peptide 5: MVALK What is the sequence of the small protein?

Reference no: EM13533164

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