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The enzyme catalase catalyzes the decomposition of hydrogen peroxide. The following data are obtained regarding the rate of reaction as a function of substrate concentration.
Run [H2O2]o (M) Initial rate (1/M·s)1 0.001 0.001382 0.002 0.002673 0.005 0.00600
The concentration of catalase is 3.5 ×10-9 M.
Use this set of data to determine the maximum rate, Vmax, the Michaelis constant, Km, and the turnover number, k2, for this enzyme.
Enzyme labeling can be used to map the structure of the active site of an enzyme using an affinity label, a molecule that is structurally similar to the substrate
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