Explain catalyzes the cleavage of phosphodiester bonds

Reference no: EM13343068

The proteins in the mixture are separated by chromatography. You have reason to believe that your unknown protein is an enzyme which catalyzes the cleavage of phosphodiester bonds. You have a solution of bis- nitrophenylphosphate (BNPP), a phosphodiester compound, which is cleaved to produce nitrophenylphosphate (NPP) and nitrophenol. NPP absorbs at 400nm. Describe how you would test the fraction from the chromatography experiment to determine if they contain the enzyme.

Reference no: EM13343068

Questions Cloud

Define the nature of the forces of attraction : What is the nature of the forces of attraction in it. Explain in terms of their structures why each of the following pairs of substances have very different chemical and physical properties

Calculate the mobility of the linkage : Compute the coordinates of the midpoint of the couple DE. Use Maple to plot the coupler curve for this point as a function of the input angle θ and calculate the mobility of the linkage

Explain the values of ka1 and ka2 for the diprotic acid : The student titrated 50.0 mL of the 0.10 M H2A with 0.10 M NaOH. After 25.0 mL of NaOH was added, the pH of the resulting solution was 6.70. After 50.0 mL of the NaOH was added, the pH of the resulting solution was 8.00. What are the values of Ka1..

What speed would the train be going : The barricade at the end of a subway line has a large spring designed to compress 2.00 m when stopping a 1.60 ? 105 kg train moving at 0.300 m/s. What speed would the train be going if it only compressed the spring 0.900 m

Explain catalyzes the cleavage of phosphodiester bonds : The proteins in the mixture are separated by chromatography. You have reason to believe that your unknown protein is an enzyme which catalyzes the cleavage of phosphodiester bonds.

Find how far apart are the half-kilogram marks on the scale : Fish are hung on a spring scale to determine their mass (most fishermen feel no obligation to report the mass truthfully). How far apart are the half-kilogram marks on the scale

Explain the enthalpy of vaporization of isobutane : Recall from the prelab the enthalpy of vaporization of isobutane. Isobutane is the fuel used in disposable lighters. The lighters' operation is dependent upon the butane's ability to vaporize and be ignited, and this depends somewhat upon the larg..

Determine the refractive index of the film : A tank of gasoline (n = 1.40) is open to the air (n = 1.00). A thin film of liquid floats on the gasoline and has a refractive index that is between 1.00 and 1.40. What is the refractive index of the film

What would be the journal entry for finished goods : what would be the journal entry for "finished goods with a carrying cost of 138500 are sold on account for 199500. assume a perpetual inventory system and enter a compound journal entry".

User Account

All Pages