Explain blue color is proportional to the amount of protein

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Reference no: EM13562020

1. In a common protein analysis, a dye binds to the protein and the color of the dye changes from brown to blue. The intensity of the blue color is proportional to the amount of protein present.

Protein (µg): 0.00(blank) 9.36 18.72 28.08 37.44
Absorbance: 0.466 0.676 0.883 1.086 1.280

a) After subtracting the blank absorbance (0.466) from the remaining absorbances, use the method of least squares to determine the equation of the best straight line through these 5 points (n = 5). Use the standard deviation of the slope and intercept to express the equation in the form
y (±sy) = [m (±sm)]x + [b(±sb)] with a reasonable number of significant figures.

Reference no: EM13562020

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