Explain blue color is proportional to the amount of protein

Reference no: EM13562020

1. In a common protein analysis, a dye binds to the protein and the color of the dye changes from brown to blue. The intensity of the blue color is proportional to the amount of protein present.

Protein (µg): 0.00(blank) 9.36 18.72 28.08 37.44
Absorbance: 0.466 0.676 0.883 1.086 1.280

a) After subtracting the blank absorbance (0.466) from the remaining absorbances, use the method of least squares to determine the equation of the best straight line through these 5 points (n = 5). Use the standard deviation of the slope and intercept to express the equation in the form
y (±sy) = [m (±sm)]x + [b(±sb)] with a reasonable number of significant figures.

Reference no: EM13562020

Questions Cloud

Find out which possesses more mass a sphere of gold : Determine which possesses more mass: a sphere of gold with a diameter of 2.00 cm or a cube of silver measuring 2.00 on each side.

Evaluate what is the angular speed of the sphere : A 240 N sphere 0.20 m in radius rolls, without slipping 6.0 m downa ramp that is inclined at 28 degrees with the horizontal. What is the angular speed of the sphere

What is the horizontal acceleration of the block : Two forces, vector F 1 and vector F 2, act on the 5.00 kg block shown in the drawing. The magnitudes of the forces are F1 = 48.0 N and F2 = 39.0 N. What is the horizontal acceleration (magnitude and direction) of the block?

Obtain the cars speed at the bottom of the dip : The passengers in a roller coaster car feel 50% heavier thantheir true weight as the car goes through a dip with a 40.0 m radius of curvature. What is the car's speed at the bottom of the dip

Explain blue color is proportional to the amount of protein : In a common protein analysis, a dye binds to the protein and the color of the dye changes from brown to blue. The intensity of the blue color is proportional to the amount of protein present.

What is the magnitude of the net force acting on the ball : When a 58-g tennis ball is served, it accelerates from rest to a speed of 44 m/s. The impact with the racket gives the ball a constant acceleration over a distance of 38 cm. What is the magnitude of the net force acting on the ball?

A pronghorn antelope has been observed to cover : A pronghorn antelope has been observed to cover 1.60 km with an average speed of 67.0 km/h. The antelopes speed during the first half of the distance is 88.0km/h. find the antelopes speed during the second half of its run

Evaluate what is the mass of the rocket : A rocket weighs 1 x10^7 N while on the surface of the earth. Theignition of its engines results in a lift off force of 1.5 X 10^7N, what is the mass of the rocket

A fluorine has a greater first ionization energy than iodine : Fluorine has a greater first ionization energy than iodine, and the reason for this is because fluorine has a greater amount of shielding of outer electrons by inner electrons than does iodine.

User Account

All Pages