Explain attraction with the stationary phase or mobile phase

Reference no: EM13251838

We did TLC and column chromatography separating methylene blue and fluorescein. The solvent contained mostly ethanol but also had NaOH, and this NaOH formed SiO- with the silica (the stationary phase), which is in competition with the solvent for interaction with analyte.

Could someone explain this competition and interaction? I know that chromatography is based on the attraction with the stationary phase or mobile phase. In this experiemnt, the more polar it is, the less Rf value is. I'm just not sure what NaOH does here and how the SiO- ion would interact with the analyte.

Reference no: EM13251838

Questions Cloud

Determine 1s complement addition and complement : Incremental Update. Typically, after every hop at least one packet header field (e.g., a packet's time-to-live field) needs to be overwritten. Because the checksum is a simple complement of a 1s complement sum, it turns out that when, for instance..

What is the mse for the eight forecasts : Which method would you suggest using to predict future sales- a linear trend equation b trend-adjusted exponential smoothing? Why?

Barbie in borderless worlds - a case study : One night a year, the attention of the TV-watching world is focused on Hollywood's most spectacular event, the Oscar award ceremony.

Options results in lower total interest costs : Which of these options results in lower total interest costs - A 30-year student loan of $120,000 with an annual interest rate of 3% or a 20-year loan of $120,000 with an upfront cost of $5,000 but an annual interest rate of 3.750%?

Explain attraction with the stationary phase or mobile phase : Could someone explain this competition and interaction? I know that chromatography is based on the attraction with the stationary phase or mobile phase

Determine the optimal solution from the output from qm : Determine the optimal solution from the output from QM for Windows.

Calculate the enthaply of formation of noctane : Calculate the enthaply of formation of noctane (C8H18) at STP from its reaction with oxygen and its ideal heat of combustion 44.9kj/g

Define what is the expected freezing point depression : If a protein sample has a molar mass of approximately 14,000 u, what is the expected freezing point depression when 1.00g is dissolved in 50.0mL of distilled water

Define what is the heat of the reaction of gaseous monatomic : What is the heat of the reaction which forms 1.00 mole of gaseous monatomic bromine from 0.500 mole gaseous diatomic bromine

User Account

All Pages