Explain apparent affinity constant for the competition assay

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Reference no: EM13274969

Using the thermodynamic cycle in the tight binding KNI-764/HIV-1 protease example discussed in class, derive the Kapp= KKNI-764/(1+KAc-pepstatin[AcPepstatin]) for the apparent affinity constant (Kapp) for the competition assay between the weak (Ac peptstatin) and tight (KNI-764) inhibitor binding to HIV-1 protease. write expressions for HIV-1 protease complexed with each of the inhibitors individually, and remember that in the competition assay, only the unbound protein can bind KNI-764

Reference no: EM13274969

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Explain apparent affinity constant for the competition assay : Using the thermodynamic cycle in the tight binding KNI-764/HIV-1 protease example discussed in class, derive the Kapp= KKNI-764/(1+KAc-pepstatin[AcPepstatin]) for the apparent affinity constant (Kapp) for the competition assay between the weak (Ac..

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