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Explain anaerobic reactions in the api 20e test

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Reference no: EM132711291

1. What piece of information about your unknown should you have prior to using the API 20E system?

2. How are aerobic reactions prepared differently than anaerobic reactions in the API 20E test?

3. Could API 20E system be used to identify the organisms in a mixed culture?

4. What type of medium could help to ensure that a Gram-positive organism was not inadvertently used to inoculate an API 20E test?

5. Why is the API 20E only useful for identifying Gram-negative rods and no other bacterial species as well?

6. The API 20E test strip has been designed to identify enteric bacteria in a clinical setting. With this in mind, design your own test strip to differentiate among the species of Staphylococcus, Streptococcus, and Micrococcus, using the flow charts presented in your Lab Supplemental Manual.

7. After inoculating all the tests in the strips, a streak-plate is often done using the same saline suspension used to inoculate the test strip to ensure a pure culture was used to inoculate the strip. What is the importance of doing this?

Reference no: EM132711291

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