Explain an open tubular chromatography column

Reference no: EM13266055

An open tubular chromatography column is 31.8 m long and has an inner diameter of 0.528 mm. The inside wall is coated with a layer of stationary phase 3.2 ?m thick. Unretained solute passes through the column in 2.23 min, while a certain solute has a retention time of 19.56 min.

(a) What are the linear and volume flow rates?
(b) What is the capacity factor, k\', and the partition coefficient, Cs/Cm, for this solute?
(c) What fraction of time did the solute spend in the stationary phase?

Reference no: EM13266055

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