Explain an enzyme-catalyzed reaction produces a product

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Reference no: EM13505465

An enzyme-catalyzed reaction produces a product that has a maximum absorbance at 412 nm. The extinction coefficient is 4000 M-1 cm-1. A 10/1 dilution of the enzyme is made and 20 mL of the dilution are put into a cuvette with 80 mL of water and 1.9 mL of reaction cocktail. The absorbance change per minute is 0.05.

a) How many units were in the cuvette if 1 unit =1 mmol/min?
b) How many units were in the cuvette if 1 unit =1 nmol/min?
c) What is the relative activity of the undiluted enzyme (1 unit = 1 mmol/min)?

Reference no: EM13505465

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