Explain aliquots of a 0.5-mg/ml standard of bsa

Reference no: EM13511596

Aliquots of a 0.5-mg/mL standard of BSA are used to construct a standard curve for the Bradford protein assay. The tubes contain the following amounts of the BSA solution: 0, 20, 40, 60, 80, and 100 µL. The corresponding absorbencies after adding Bradford reagent are 0, 0.05, 0.09, 0.14, 0.19, and 0.22. If you take 20 µL of an unknown and add 80 µL of water, mix, take 10 µL of the mixture and add to Bradford reagent, and see an absorbance of 0.08, what is the protein concentration of the undiluted unknown?

Reference no: EM13511596

Questions Cloud

Result of enron and other corporate auditing failures : If accounting were not regulated, we would not be facing the difficult problems that have arisen as a result of Enron and other corporate auditing failures. Do you agree with this statement?

Calculate the total work done on the crate : A factory worker pushes a 30.0kg crate a distance of 4.4 m along a level floor at constant velocity by pushing downward at an angle of 31 below the horizontal. What is the total work done on the crate

What is the economic order quantity : What is the economic order quantity? Should a small business establish a method of inventory management? Why or why not? What steps would be involved for the small business to set up inventory management?

Implications for information production : Why does accounting information have some features of a public good? What are the implications for information production in both unregulated and regulated markets?

Explain aliquots of a 0.5-mg/ml standard of bsa : Aliquots of a 0.5-mg/mL standard of BSA are used to construct a standard curve for the Bradford protein assay. The tubes contain the following amounts of the BSA solution: 0, 20, 40, 60, 80, and 100 µL.

How much work is done on the crate by this force : A factory worker pushes a crate of mass 32.0kg a distance of 4.85m along a level floor at constant velocity by pushing horizontally on it. How much work is done on the crate by this force

How has sox affected problems : Why have management consulting operations created problems for the public accounting industry? How has SOX affected these problems?

By how much does it have to increase its speed : Suppose a satellite is in a circular orbit 5.175 Earth radii above the surface of the Earth, By how much does it have to increase its speed

9 different clinical professions : 9 different clinical professions

User Account

All Pages