Explain absorbance far outside the linear region

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Reference no: EM13160289

A solution (S1) of yellow # 5 has an absorbance far outside the linear region of Beer's law. In order to determine the concentration of this solution, a serial dilution is performed. To make so elution S2, 20 mL of S1 is diluted to 50 mL. To make solutionS3, 25 Ml od S2 is diluted to 100 mL. The absorbance of S3 is measured to be 0.714 at 430 nm using a 1 cm curvette. Use the true value of e and calculate the concentration of solution S1.

Reference no: EM13160289

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