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The transformation was successful, however, when my plate with 10 uL did not show any colony growth, whereas, my 200 uL plate showed colony growth. Both the E. Coli came from the same culture but I am not sure why the lower concentration of E. coli + plasmid did not show any colony growth. Can someone explain a possible source of error for this?
The top of mountain is at an elevation of 45° from one bank of a river and at an elevation of 60° from the other bank. If river has a width of 1 km, what is the height [in km] of the mountain.
Will xylem fluid flow into the aphid. Which microbes are most susceptible versus most resistant to disinfectants? Why.
According to ABC model of floral development, which genes would be expressed in a showy ornamental flower with multiple sepals and petals but no stamens or carpels.
Phenylketonuria, PKU, is a neurodegenerative disorder resulting from inability to metabolize phenylalanine. PKU is inherited as autosomal recessive trait.
Is habitat temperature reflected in the genomes? What is presumably connected to thermostability.
List two types of macromolecules that have been partially digested by the time acid chime moves into the intestine. Where did the digestion take place, and what enzymes were involved.
When did dinosaurs and pterosaurs become extinct? Which are the only extant animals that descended directly from dinosaurs.
how to Calculate the fraction of Vmax. For an enzyme which obeys Henri-Michaelis-Menten kinetics.
however normal E.coli cells can synthesize all the amino acids, some mutants called amino acid auxotrophs, are unable to synthesize specific amino acids and require the addition of that amino acid to the culture medium for optimal growth.
Who was Carl Weigert? What influence did Weigert have on Ehrlich. Other than the acid-fast stain, what other technique may be used to diagnose tuberculosis? What scientist developed this test.
What specific biotechnology application did you choose. What benefits are derived from this application.
A sunflower population in Hardy-Weinberg equilibrium has the given allele frequencies for petal color: 0.75 dominant, 0.25 recessive.
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