Reference no: EM13240709
Consider the peaks for pentafluorobenzene (12.98min) and benzene(13.20min) in the chromatogram shown above. The elution timefor the unretained solute is 1.06 minutes. The open tubularcolumn is 30.0m in length and 0.530mm in diameter, with a layer ofstationary phase 3.0um thick on the inner wall.
A) Find the adjusted retention times and capacity factors for bothcompounds.
B) Find the relative retention, ?.
C) Find the separation factor, ?.
D) Measuring w1/2 on the chromatogram (0.13min forpentafluorobenzene and 0.15min for benzene), find the number ofplates, N1 and N2, and the plate height forthese two compounds.
E) Measuring at the width, w, at the baseline on the chromatogram(0.28min for pentafluorobenzene and 0.30min for benzene), find thenumber of plates for these two compounds.
F) Use your answer to part E to find the resolution between the twopeaks.
G) Using the number of plates N = ?N1N2with values from part E and the observed separ
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Explain a layer ofstationary phase thick on the inner wall
: Consider the peaks for pentafluorobenzene (12.98min) and benzene(13.20min) in the chromatogram shown above. The elution timefor the unretained solute is 1.06 minutes. The open tubularcolumn is 30.0m in length and 0.530mm in diameter, with a layer ..
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