Reference no: EM133199555
Experiment 1: Testing for Biomolecules
Part 1: Testing for Proteins
1. Take a photograph of your four labeled test tubes at the end of your experiment (step 9). Your photograph should include your name and date in the image.
INSERT PHOTOGRAPH HERE
2. Record your experimental results in the following table.
|
Tube
|
Initial Color
|
Hypothesis (Protein +/-)
|
Final Color
|
Protein Present?
|
|
1
|
light yellow
|
|
purple
|
yes
|
|
2
|
light yellow
|
|
purple
|
yes
|
|
3
|
clear
|
|
light blue
|
no
|
|
4
|
clear
|
|
light blue
|
no
|
Part 2: Testing for Reducing Sugars
3. Take a photograph of your four labeled test tubes at the end of your experiment (step 13). Your photograph should include your name and date in the image.
INSERT PHOTOGRAPH HERE
4. Record your experimental results in the following table. (5 points)
|
Tube
|
Initial Color
|
Hypothesis (Reducing Sugar +/-)
|
Final Color
|
Reducing Sugar Present?
|
|
1
|
white
|
|
greenish blue
|
yes
|
|
2
|
white
|
|
green
|
yes
|
|
3
|
clear
|
|
blue
|
no
|
|
4
|
clear
|
|
blue
|
no
|
Part 3: Testing for Starch
5. Take a photograph of your four labeled test tubes at the end of your experiment (step 9). Your photograph should include your name and date in the image.
INSERT PHOTOGRAPH HERE
6. Record your experimental results in the following table.
|
Tube
|
Initial Color
|
Hypothesis (Starch +/-)
|
Final Color
|
Starch Present?
|
|
1
|
clear
|
|
yellow
|
|
|
2
|
clear
|
|
yellow
|
|
|
3
|
clear
|
|
purple
|
|
|
4
|
clear
|
|
clear
|
|
Part 4: Unknown Sample
7. What types of biomolecules do you hypothesize are present in your unknown sample?
8. What were your positive and negative controls for each biomolecule when testing the unknown? Explain why you chose each control.
9. Take a photograph of your labeled test tubes at the end of your protein testing. Your photograph should include your name and date in the image.
INSERT PHOTOGRAPH HERE
10. Take a photograph of your labeled test tubes at the end of your reducing sugars testing. Your photograph should include your name and date in the image.
INSERT PHOTOGRAPH HERE
11. Take a photograph of your labeled test tubes at the end of your starch testing. Your photograph should include your name and date in the image.
INSERT PHOTOGRAPH HERE
12. Create data tables to display your results from your experiments.
INSERT TABLES HERE
13. What can you conclude about the unknown sample based on your results?
Here are some helpful tips where students tend to lose points on this lab -
- Include the correct images in the correct places - notes what steps need images BEFORE you start.
- Testing for proteins, starch, and glucose ARE DIFFERENT PROCESSES. Follow the directions carefully.
- In the tables in #2, #4, and #6 - you should have results for all 4 rows.
- In #7, a hypothesis should be a detailed educated prediction, for example - "If I test the unknown solution for proteins, starch, and glucose, the unknown solution will test positive for _______________."
- In #8, you need a positive and negative control for protein, starch, and glucose. Select your controls from your results in the first half of the lab where you tested for proteins, starch, and glucose. Format your answer as follows:
- In #9-11, you should have three test tubes in each image. The positive control, the negative control, and the unknown. You do NOT just go by the results from the first half of the lab, you have to include your controls in each step to ensure you did the step correctly. The positive should test positive, and the negative should test negative, if it doesn't, you have made a mistake.
- In #12, you will have three tables. Format your tables like the ones in the biology lab text - here is a site that might help you if you do not know how to create tables. This is a skill you will need throughout the term https://computer.howstuffworks.com/how-to-make-table-on-microsoft-word.htm
- In #13 - be very thorough. DO NOT JUST STATE "The unknown solution is a protein." I want to know how you came to that conclusion based on your experimental results in the table. Reference the controls and variables.
- Protein positive control: _______________ - because ________________________________.
- Protein negative control: _______________ - because _________________________________.
- Starch positive control: _______________ - because _________________________________.
- Starch negative control: _______________ - because _________________________________.
- Glucose positive control: _______________ - because _______________________________.
- Glucose negative control: _______________ - because _______________________________.