Reference no: EM133142274
Experiment - Determination of the relative proportions of caffeine and aspirin in proprietary analgesics by gas chromatography-mass spectrometry (GC-MS)
In this experiment you will be expected to determine the proportion of caffeine and aspirin in a proprietary analgesic.
Standards - Students are provided with 1000 ppm solution of caffeine and 1000 ppm ethyl-2-acetoxybenzoate. Using the 10 mL volumetric flasks provided, prepare solutions containing both analytes over a range of 50 to 250 ppm concentrations, diluted in methanol.
Analysis - Fill the auto sampler vials ¾ full with each sample. A method file will be set up for your use and you will be shown how to set up a sequence file to run the samples required to obtain a quantitative calibration and to analyse the mixture of analytes. Analyse the standards once only and the unknown sample three times.
When each of the sample solutions has been analysed you will use the software to obtain peak area and height measurements to enable you to construct appropriate calibration graphs and to determine the amounts of caffeine and derivatised aspirin in the unknown solution.
The procedure will consist of obtaining the total ion current trace for each of the analyses and then analysing these using the "peak detect" software which will determine the area and height of the signal from each of the analytes.
Report - Complete and upload your audio-visual presentation as instructed.
Attachment:- Experiment - Determination of the caffeine File.rar