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Exonucleolytic proofreading step

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The accuracy of DNA polymerase activity is subject to a 3′ exonucleolytic proofreading step. Which of the following statements is true?

  • The polymerase and exonuclease site are physically close to each other.
  • The 3′ exonuclease has very high specificity for mismatched pairs.
  • The K M for DNA polymerase for chain extension from a mismatch is about 3 orders of magnitude higher, allowing the mismatch to reach the exonuclease site.
  • All of the listed statements are true.

Reference no: EM131371171

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