Reference no: EM132785760
1. The protozoa, algae and S. cerevisiae are eucaryotes, E.coli is a procaryote. Based on your observations, what generalizations can you make about eukaryotic and prokaryotic cells?
2. The bacteria and yeast you observed are about 2 to 10 micrometers in length and diameter. Based on these known sizes, estimate the average size of some of the protozoa and algae
3. A student missed the laboratory period where use of the microscope was demonstrated. The instructor asked the student to read the description in the laboratory manual and then proceed to examine bacterial cells with the oil immersion lens. The student skimmed the directions and began. After about 15 minutes of struggling, the student gave up in despair without seeing anything. Below is a detailed description of what student did. How many mistakes did the student make and why didn't the student see anything?
a. Plugged in the microscope and turned the light source to maximum intensity.
b. Make a wet mount and placed it on the stage with the low power objective lens in position.
c. Tried to focus with the coarse adjustment knob, but decided the bacteria were too small and needed to be seen with the high power objective lens
d. Rotated the high power objective lens into position, but saw that the lens would likely touch the slide, so lowered the stage so that the objective lens rotated freely
e. Tried to focus with the coarse adjustment knob, but still saw nothing.
f. Decided to switch to the oil immersion lens, but still saw nothing.
4. Look at the objectives on your microscope. What can you say about the diameter of each lens and its relationship to the magnifying power of the objective.
5. Is there observation can be make in any striking differences between the phase and bright field images of the various cells? What are they?
6. Suppose we did not observe a distinctly different image with the phase objective. What might it mean?
7. Give 3 examples of organisms or phenomena for which observation by phase microscopy might be preferable to bright-field
8. Would phase microscopy be of any particular value when examining a stained specimen? Explain
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