Examine the expression of the cd4 marker

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Reference no: EM132553233

To examine the expression of the CD4 marker on T cells using a CD4-FITC antibody in flow cytometry, which gating would we use?

  • gate the T cells based on low FSC and SSC and then examine FITC levels on these cells
  • gate on positive detection of FITC and then examine forward and side scatter (FSC/SSC) properties
  • gate on negative detection of FITC and then examine FSC/SSC properties
  • gate the T cells based on high FSC and SSC and then examine FITC levels on these cells
  • measure the FITC levels on all circulating leukocytes

Reference no: EM132553233

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