Evaluating expression of twist and snail

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Your colleague is perplexed - he tells you that he's monitoring the EMT in the mice by evaluating expression of Twist and Snail (known to be required for the EMT), which do increase as he observes the tumor cells breaking through the basement membrane of the colon epithelium. He's looking at their expression via immunoblots. But he's confused because b-catenin levels stay about the same and he expected them to increase. You reassure him that this observation is consistent with the EMT. What is your explanation and what simple experiment can you suggest that would demonstrate your explanation?

Reference no: EM132548029

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