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The hydration of CO2, CO2 + H2O ? HCO3 - + H+, is catalyzed by the enzyme carbonic anhydrase. The steady-state kinetics of the forward (hydration) and reverse (dehydration) reactions at pH 7.1, 0.5°C, and 2 mM phosphate buffer was studied using bovine carbonic anhydrase. Some typical results for an enzyme concentration of 2.3 nM are Hydration Dehydration 1/v c(CO2) 1/v HCO3 - 36 · 103 1.25 95 · 103 2 20 · 103 2.5 45 · 103 5 12 · 103 5 29 · 103 10 6 · 103 20 24 · 103 15 Make suitable plots of data and determine the Michaelis constant KM and the rate constant k2 for the decomposition of the enzyme-substrate complex to form product for a. The hydration reaction. b. The dehydration reaction. c. From your results, calculate the equilibrium constant for the reaction CO2 + H2O ? HCO3 - + H+ (Hint: Start by writing an equation that defines the equilibrium condition in terms of the Michaelis-Menten formulation of this reaction. Note that the kinetics was measured at pH 7.1.)
How many different aldols (b-hydroxyaldehydes), including constitutional isomers and stereoisomers, are formed upon treatment of butanal with base?
At 720'c the equailibrium constant Kc for the reaction below is 2.37*10^-3. If the constant of materials is [N2]=0.638M [H2]=8.80M and [NH3]=3.65M, predict if the equilibrium will have a net reaction to the right(toward products) or to the left(to..
How do the requirements that t and v are constant enter the derivation for delta Asys
What is the initial temperature of a gas if the volume changed from 1.00 L to 1.10 L and the final temperature was determined to be 255.5oC
Carbon monoxide detectors sound an alarm when peak levels of carbon monoxide reach 100 parts per million (ppm). This level roughly corresponds to a composition of air that contains 400,000 micrograms per cubic meter of air.
the difference between nucleophilic centers, nucleophiles, electrophilic centers, and electrophiles. Lastly, tell me how to identify all four of these in a given molecule.
What is the iron molar concentration in the serum sample? Not that the following relationship may be used: Aunk/Aunk+std - Cunk/{Cunk+Cstd}
If 153 mL of wet H2 is collected over water at 24°C and a barometric pressure of 725 torr, how many grams of Zn have been consumed
The silicon in the plate is a mixture of naturally occurring isotopes. One of the those isotopes is silicon-30, which has an atomic mass of 29.97376 and a percent natural abundance of 3.10.
What is the pressure in a 1.00 Liter container of methane, CH4, that contains 40.0g of the gas at 25.0 degrees Celcius
In C7H8BrNO there are 4 degrees of saturation. Will an open chain structure with 2 triple bonds exist? Open chain with 2 triple bonds. Is this incorrect. Is it too unstable to exist or is it possible.
What are some possible sources of error in your calculated atomic weight for Mg? A. Calculate the % Absolute error. B. Calculate the % relative error in the mass, volume, temperature, and other measurements.
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