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Look up the pKas of glutamic acid and draw thestructures for the predominant forms of glutamic acid at pH 1.0, pH3.0, pH 7.4, and pH 12. Examine the structures and determine thenet charge of glutamic acid at each of these pHs. The pH at which the predominant form of an amino acid has a net of 0 is known asthe pI. Estimate the pI of glutamic acid.
Compare and contrast GEFs. GAPs, and GDIs in cell signaling processes. Give three different examples of when these proteins might be used.
how a mutation in a DNA exon will change the eventual mRNA sequence, and how this may or may not change the amino acid sequence.
Estimate which type of protein binds to improperly folded or improperly assembled proteins in the ER, holding them there until proper folding occurs?
You measured the purified protein concentration, which is 5 ug/uL. You decided to load 20 ug protein to the gel. How would you dilute your sample?
Prions are causative agents of progressive neurodegenerative disorders that affect both humans and animals. Like viruses, prions are acellular,
What is the metabolic exchange between rhizobia and their plant hosts? What does the plant provide in addition to carbon and how does leghemoglobin play a role in this? What metabolic feature of the bacterium is useful to the plant.
Discuss the three theories about the evolution of multicellularity, giving supportive examples of each. Include in your discussion the strengths and weaknesses of each theory.
Explain how epithelium is classified and discuss at least three functions of the tissue type.
What are the components of a DNA nucleotide? Why was it that knowing the composition of DNA led most scientists to the mistaken belief that DNA could not be the genetic material?
What information would you get from a western blot that you would not get from a northern blot when used to analyze the expression of the same gene?
The 4X objective has a field of view of 3mm. If you viewed a specimen through the 10X objective and the specimen spanned approximately 50% of the field of view!
Which type of succession takes longer? a) primary succession or b) secondary succession?
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