Estimate the molecular mass of unknown protein

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Questions

1. What is the order of elution of the following proteins from a Sephadex G-50 (gel filtration) column: catalase, alpha-chymotrypsin, concanavalin B, lipase, and myoglobin?

2. Estimate the molecular mass of an unknown protein that elutes from a Sephadex G-50 (gel filtration) column between cytochrome c and ribonuclease A.

Reference no: EM133458533

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