Estimate the highest resolution of subcellular structures

Reference no: EM133319127

Case: You are interested in imaging an EGFP-tagged protein (lex = 488 nm, lem = 509 nm) located on the surface of a cell's mitochondrion.

Question 1: Using a 100x NA1.4 objective lens, estimate the highest resolution of subcellular structures that you can expect to achieve using conventional (non-confocal) fluorescence microscopy?

Question 2: In a futile attempt to double your image resolution you increase the magnification of your eyepieces from 10x to 20x. To your surprise you find that image resolution is degraded instead. Why does this happen?

Reference no: EM133319127

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