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Sample Purified histidine tagged FABP by affinity chromatography using agarose beads with chelated nickel (Ni-NTA, Ni-nitrilotriacetic acid). samples were run on pre-made sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). Use one gel for Western Blotting transfer on Polyvinylidene difluoride (PVDF) membrane gel was stained with Coomassie blue for all proteins
Referring to your figures, identify the his-FABP5 protein and estimate its molecular weight on the gel and western blot, and compare it to the value that you would expect given that the approximate molecular weight of FABP5 plus the his-tag sequence HHHHHHSSGLVPRGS. Discuss the purification in terms of the four fractions that you analyzed and estimate the fold purification from the position and intensity of the bands in the different fractions.
It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.
This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.
Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.
This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.
Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?
Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.
Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.
The use of PCR and genetic approaches in biotechnology
Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.
What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?
Prepare an essay on nosocomial infection.
To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.
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