Epithelial tissues during primary cell culture

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Reference no: EM1383788

Question: Explain why does depletion of Ca2+ from the medium by chelating agents promote isolation of cells from epithelial tissues during primary cell culture?

A) Given the following amounts added to make 500ml of a given solution, what are the final concentrations.

2.5 ml of 2M Tris, pH 7.5

4.4 grams of NaCl (MW=58.44)

500ul of 100% Tween-20

B) How much would be required to make 4 liters of the same solution.

Reference no: EM1383788

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