Epithelial tissues during primary cell culture

Reference no: EM1383788

Question: Explain why does depletion of Ca2+ from the medium by chelating agents promote isolation of cells from epithelial tissues during primary cell culture?

A) Given the following amounts added to make 500ml of a given solution, what are the final concentrations.

2.5 ml of 2M Tris, pH 7.5

4.4 grams of NaCl (MW=58.44)

500ul of 100% Tween-20

B) How much would be required to make 4 liters of the same solution.

Reference no: EM1383788

Questions Cloud

Determine the allele frequency for dominant allele : Phenylketonuria is an autosomal recessive genetic disorder. The frequency of homozygotes with this disorder is 0.0001. Determine the allele frequency for the dominant allele?

What are the responsibilities of businesses in industrialize : What are the responsibilities of businesses in industrialized nations to businesses in developing nations? Are there any? Why? Why not?

Employers and employees have a unique relationship : Do you believe Pam is acting unethically? What about Dunder Mifflin? Explain please? Image text transcribed for accessibility: Employers and employees have a unique relationship

Test of a driver perception reaction time : A test of a driver's perception reaction time is being conducted on a special testing track with wet pavement and a driving speed of 55 mi/hr. When the driver is sober, a stop can be made just in time to avoid hitting an object that is first visib..

Epithelial tissues during primary cell culture : Explain why does depletion of Ca2+ from the medium through chelating agents promote isolation of cells from epithelial tissues during primary cell culture?

Universal functions of religion : Critically discuss the four universal functions of religion and the importance of each of these to human beings and social relationships. Can we have these relationships without religion?

Making reaction : Assume you are given a primer with a concentration of 1.4 mg/ml, RNA concentration of 0.8 mg/ml, 1M MgCl2, 10mM dNTP mix, 10x buffer, 10 units/ul of reverse transcriptase.

Explain application architecture and process design : Explain the application architecture and process design.Create the physical data flow diagrams. Use Microsoft Visio to make examples of flow charts and data flow diagrams.

What is the total area of the surface : What is the length of BF, in centimeters and how many exams has Jeff taken so far and what is the total area of the surface of the cube that is NOT red?

User Account

All Pages