Reference no: EM133241383
Procedure I - Enzyme Reaction Rate - Concentration Dependence
Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
|
Trial
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Concentration Level
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Oxygen Concentration Change Data (ppt)
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Elapsed Time (s)
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Enzyme Reaction Rate (ppt/s)
|
|
1
|
2.5%
|
5.64
|
20.0
|
|
|
2
|
5.0%
|
11.28
|
20.0
|
|
|
3
|
10%
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22.56
|
20.0
|
|
Observations and Questions
[1] Sample Calculation: Show your enzyme reaction rate calculation for Trial 1.
[2] Convert enzyme concentrations of 2.5%, 5.0%, and 10% to ppt units.
[3] For Procedure I/Table I, identify:
Independent variable:
Dependent variable:
Control variables:
[4] Draw a graph of the independent variable on the x-axis (abscissa) and the dependent variable on the y-axis (ordinate). Label the axis titles and units.
Procedure II - Enzyme Reaction Rate - Temperature Dependence
Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
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Trial
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Temperature (°C)
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Oxygen Concentration Change Data (ppt)
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Elapsed Time (s)
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Enzyme Reaction Rate (ppt/s)
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|
1
|
20.0
|
7.95
|
20.0
|
|
|
2
|
30.0
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16.01
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20.0
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|
|
3
|
55.0
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2.49
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20.0
|
|
Observations and Questions
[5] Sample Calculation: Show your enzyme reaction rate calculation for Trial 1.
[6] Using the data you reported in the Table above, describe the effect of temperature on the reaction rate.
Procedure III - Enzyme Reaction Rate - pH Dependence
Complete the table below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
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Trial
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pH Level
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Oxygen Concentration Change Data (ppt)
|
Elapsed Time (s)
|
Enzyme Reaction Rate (ppt/s)
|
|
1
|
6.0
|
6.85
|
20.0
|
|
|
2
|
7.0
|
11.28
|
20.0
|
|
|
3
|
9.0
|
1.52
|
20.0
|
|
Observations and Questions
[7] Sample Calculation: Show your enzyme reaction rate calculation for Trial 1.
[8] Choose either a bar graph or a line graph format and plot the data for Procedures II and III. Plot the data for each Procedure on a separate plot.
[9] Compare the plot of the data for Procedures I, II, and III. Describe the differences among the three plots.
[10] What conclusions can draw about the effects of concentration, temperature and pH on the reaction rate after comparing the plots from the three different Procedures?
[11] Choose one of the factors that impacts reaction rate (concentration, temperature, or pH) and, in your own words, provide a plausible biological explanation for your experimental results for that factor.