Enzymatic activities of prokaryotic DNA polymerase

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Reference no: EM133201262 , Length: 2 Pages

1. Describe the 3 enzymatic activities of prokaryotic DNA polymerase I.

2. Describe in detail the role of prokaryotic DNA polymerase III.

3. Explain in detail why DNA polymerases require a primer to extend nucleotide chains.

4. Describe in detail the protein structure of DNA polymerase I; include in your discussion the Klenow fragment of DNA polymerase I.

5. Describe in detail the prokaryotic DNA polymerase III subunit interactions that prevent the enzyme falling off the DNA strand during its activity.

6. In nucleotide excision repair in E. Coli, the key enzymatic complex is the ABC excinuclease; what is an excinuclease and what are the functions of the three subunits of the above named complex? What about the nucleotytic activity of the ABC excinuclease is novel?

Reference no: EM133201262

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