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A mixture of two drugs and their metabolites were analysed by reverse phase HPLC on a 50 mm ODS column by isocratic elution using a mobile phase of 50% methanol / 50% aqueous 1% acetic acid delivered at a flow rate of 1.5 mL/min. The column dead volume was measured as 0.30 mL and a column plate height (H) of 0.02 mm was determined.
Calculate the elution time in minutes of an unretained solute (tm) and the average linear velocity (ux, mm/s) of the mobile phas
The mobile phase in the above analysis is 50% methanol / 50% aqueous 1% acetic acid. Firstly, it is changed to 40% methanol / 60% water (with 1% acetic acid) and then, secondly, to 50% acetonitrile / 50% water (with 1% acetic acid) instead of the original mobile phase in a series of additional chromatography experiments. All other experimental conditions remain the same. Explain (3-6 sentences using key words to describe the chemistry) the effect that each change will have on:
1. the chemical nature of the mobile phase,
2. interaction of the different solute types with the stationary phase, and
3. the retention times of the two peaks due to drugs A & B in each instance
Depict the structure(s) of the epoxide(s) you would get by formation of a bromohydrin from cis-3-heptene, followed by treatment with base
The following data were obtained when a Ca2+ ion-selective electrode was immersed standard solutions whose ionic strength was constant at 2.0 M.
an ethanol-water mixture is to be flash distilled at 1 atm. if the feed is 25 mole % ethanol, what are the liquid and vapor compositions if
how many regions of high electron density are there around the central atom of the molecular structure of SbCl3.
When heated, all metallic hypochlorites decompose and produce oxygen. Considering this, what happens when the decomposition reaction occurs at an incident involving fire? Please reference your answer and tell me why.
metal sample weighing 37.6 grams which has been heated to a temperature of 100 degree c in boiling water, was placed in 48.6 grams of water in a calorimeter, the inital temperature of the water was 25.2c at equilbrium the temperature of the water ..
The enthalpy change for the oxidation of styrene, C8H8, is measured by calorimetry. C8H8(l) + 10O2(g) ? 8CO2(g) + 4HOH(l) ?rH = -4395.0 kJ/mol How much heat in kJ is required to oxidize 25.0 g of styrene?
undergo an isothermal compression until the pressure of the system is four times the original pressure. The process may occur in two ways:
The products include Cr3+ ion and I2. What was reduced in the reaction and what was the reducing agent?
At 20 degrees Celcius and 1 atm (pressure), gold (Au) has a density of 19.32 g/cm cubed. What would be the volume of 2.510 kg of gold at the same temperature and pressure
A mixture of 0.10 mol of NO, 0.050 mol of H2 as well as 0.10 mol of H20 is placed in a 1.0L vessel at 300K. The subsequent equilibrium is established 2NO(g)+2H2(g) N2(g) and 2H20(g) at equilibrium [NO]=0.062M.
Calculate the time needed for the amount of ethane to fall from 1.43 10-3 mol to 2.00 10-4 mol in a 500. mL reaction vessel at 700.°C.
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