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Elution buffer composed

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How to prepare 100 mL of elution buffer composed of: 20 mM Tris, 300 mM NaCl, and 500 mM Imidazole. Advise the student on how to make a 100 mL of elution buffer (20 mM Tris, 300 mM NaCl, and 500 mM Imidazole) using water as a solvent. NP: molecular weight of each component is as follows: Tris - 121.14 g/mol, NaCl - 58.44 g/mol and Imidazole - 68.08 g/mol. Show your calculations.

Reference no: EM133261399

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