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Unlocked one of your documents and this is one of the questions i had a question about it. If a double-stranded circular DNA molecule has 4 recognition sites for HindIII, how many fragments will be generated if you digest that molecule with HindIII? How many fragments will result if you linearize the above ds DNA molecule and then digest with HindIII? Explain the answer that you had was--> A ds circular DNA with 4 sites for a restriction enzyme will generate four fragments because one of the sites will linearise it. A linear DNA will give 5 fragments. --> Can you explain it in detail as to how you obtained it? If you can give a step by step how you got the fragments and linear DNA fragments? and the other question i had was this one--If you are tasked with screening a genomic library for a specific DNA sequence and a cDNA expression library for the expression of a specific protein, what kind of screening methods will you use for each and why? the answer you provided for this one was --> A cDNA library can be screened for a particular protein using the protein fragment complementation assay (PCA) technique using Green Fluorescent Protein (GFP) as a reporter. Interactions of a 'bait' protein of interest and 'prey' cDNA fragment brings the fragments of the "reporter" protein in close enough proximity to allow them to form a functional reporter protein whose activity can be measured...How did you get this answer? I have looked all over the book and notes and have yet to get that. Please help. any advice or feedback would be greatly appreciated.
The researchers concluded that at some time in the past, a slug ate algal cells and their DNA was somehow incorporated into the slug's DNA. This process is called horizontal gene transfer and may have happened numerous time in many different speci..
1 describe the demographic transition model and how it was developed by demographers.2 describe the 4 phases of
Data from enzyme kinetic studies of an enzyme at specific substrate concentration. Data below also include from studies with town different inhibitors.
A biologist was testing E. coli for thymidine requirement. She found a thy- point mutant and wanted to study reversion to thy+. She grew 20 cultures of the thy- mutant strain.
Three Hfr strains of E. coli are known to transfer their genetic material during conjugation in different sequences. Given the time of entry of the markers into the F- recipient,
question 1 describe the characteristics of each type of muscle. how are they the same? how are they different? why are
Explain what is meant through a negative feedback loop and how these work in terms of regulating a metabolic pathway. provide an example of a negative feedback loop in the glycolytic pathway.
Assume you treated butter with a fatty acid desaturase, an enzyme that removes hydrogen from fatty acids and creates double bonds.
consider a locus with 20 alleles, each equally frequent. Under hardy-weinberg equilibrium, calculate the frequency of heterozygotes (as a whole) and homozygotes (as a whole) in the population.
How do axons develop and form connections within the brain? What difference exist between synapse formation in the CNS and in neuromuscular junctions?
He lacked the proper instruments for determining Na+ concentrations, but was rather certain that no other major ions were present in the ECF and ICF. Can you calculate either concentration for him? (Show all calculations)
whats the class of warm blooded, usually hairy animals, thats feed their young with milk by mothers? This class includes monotremes, marsupials, primates, cats, dogs, bears, hoofed animals, rodents, bats, seals, dolphins, and whales.
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