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These are questions from Western Blotting Lab. Please answer any question you know!
1)Why do proteins travel through the gel to the cathode?2)What might a given lane look like upon Coomasie staining?3)Which migrates farther, smaller protein or larger proteins?4)Draw a schematic of the transfer "sandwich".5)What are the qualities that of PVDF membrane that make it ideal for blotting?6)How do the proteins get transferred from the gel to the membrane?7)What is the importance of blocking step?8)Draw a schematic of an antibody molecule and lable its antigen binding sites and it conserved sites.9)What are primary antibodies specific for?10)What are secondary antibodies specific for?11) How are antibodies produced?12) How does the detection method work?
What is meant by a "stable phenotype" for cells? How can one go about trying to ensure a stable phenotype in vitro?
A company has designed a new weight loss drug. They want to determine if the drug is carcinogenic, so they use it in the Ames test.
A patient was hospitalized for a surgical process requiring general anesthesia. After surgery, it was noted that there were few to no bowel sounds and her bladder was not emptying as it should.
A student analyze 5,000 RFUs in a 200ul aliquot from a G3-500 ml culture. How many total RFUs will be present in a G3-15ml sample? During rGFP purification in lab#4.
An individual has been bought to you with the symptoms of Klinefelter syndrome, although a karyotype analysis indicates they have forty-six chromosomes.
Sudden addition of oxygen (O2) to a previously anaerobic culture of yeast fermenting grape juice results in a dramatic decrease in the rate of glucose consumption. This is known as "Pasteur effect".
you wish to insert a human gene into bacterial DNA.Why would you use same enzyme to cut the human DNA as you selected to cut the bacteria DNA above? please only those who know the details.
In an experiment to look at length of cell cycle phases, cells were first treated with colchicine to arrest them at mitosis. What cytoskeletal element is inhibited by colchicine to cause this arrest?
In a fern's life cycle, of hybrid "1" the sperm in the antheridium was found to have a total of 8 chromosomes. On one of the chromosomes was the dominate gene "T" on one of the chromosomes
Why is his discovery important for modern-day molecular biology?
Briefly describe how the oxygen effect plays an important role in reducing the ability of a radiation oncologist to treat tumors with the high energy electron beams from accelerators in Radiation Oncology facilities.
A 100 ml culture bottle is filled with LB (Lysogeny broth) which has all the nutrients and plenty of oxygen to support of oxygen to support E. coli growth. By performing serial dilutions and plate count you estimated that there are 5x10^5 cells pe..
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