Draw a restriction map based on the fragments

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Reference no: EM13196780

Q1. Design pentamer (5 nucleotide) primers that would amplify the entire DNA sequence shown here.

5'-ATTCGGATTACATCGGCATTACCGATTTAAAGCCCTTAACG-3'

3'-TAAGCCTAATGTAGCCGTAATGGCTAAATTTCGGGAATTGC-5'

Q2. Draw a restriction map based on the fragments resulting from the following restriction digestions of a 3 kb plasmid

Enzyme Fragment size (kb)

EcoRI 1.0; 2.0

BamHI 1.3; 1.7

EcoRI + BamHI 0.4; 0.6; 0.7; 1.3

Reference no: EM13196780

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