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For an enzyme (5 µM) , the following initial velocities have been reported depending on the substrate concentration:
[Substrate], mM v0, mM s-10.02 10.830.04 18.570.07 26.760.1 32.500.15 39.000.2 43.330.3 48.750.5 54.170.7 56.88
(a) Draw a Michaelis-Menten plot for this enzyme.(b) Draw a Lineweaver-Burke plot for this enzyme.(c) Determine KM and vmax for this enzyme(d) Indicate in both graphs (a & b) where vmax and KM can be recognized.(e) Calculate the dissociation constant for this enzyme.(f) Calculate the efficiency of this enzyme.
Blindness is an autosomal dominant disorder in puppies. B or b are used to represent the gene that causes blindness. Black Hair Follicular Dysplasia is an autosomal recessive disorder in puppies.
Suppose an analyzer created a purebred strain of these smart mice and started breeding them with other mice to map the gene responsible for the phenotype.
Retinoblastoma is an eye cancer caused by a dominant allele. A normal female and an affected male have four children: one normal and three affected.
Sarah, a twenty year old female premed student is rushed into the emergency room with complaints of fainting, seizures, fever, a headache and weakness.
You have an F- strain of E. coli that is resistant to strepto- mycin (Str(r)) but requires the following amino acids for growth on minimal medium: arginine, cysteine, methionine, phenylala- nine, and proline.
What is the significance of adenine, thymine, guanine, and cytosine in the structure of a DNA molecule.define how these four nucleotides bond.
How do both meiosis and sexual reproduction (fertilization) produce offspring that differ genetically from the parents? Be sure to talk about the two specific steps in meiosis that increase variability as well as the process of fertilization.
Among various factors that influence enzyme activity, phosphorylation is one of the most common mode of regulation.
Assume a male patient is searching for the cause of his infertility. A karyotype shows he has two "X" chromosomes, but otherwise normal chromosomes.
Who does not readily enter blood capillary of the villi but must enter the lymphatic capillary of villus instead, amino acid, gluclose, calcium and copper or cholesterol and triglyceride?
Compare the recipes of nutrient agar and MacConkey agar. If an organism can grow on both media,on which would you expect it to grow better?
The trees derive no known benefits from the dwarf mistletoes but appear shorter than trees not associated with dwarf mistletoe. Which is the best describes the interactions between dwarf mistletoes and trees.
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