Disrupts the quaternary structure of a protein

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A particular compound, compound X, completely disrupts the quaternary structure of a protein but has no effect on its primary, secondary, or tertiary structure.

A. Treatment with excess compound X should break down the protein completely into (a) free amino acids (b) short peptides 10-20 amino acids long (c) individual peptide chains (d) individual molecules of protein each containing several peptide chains (e) can't predict

B. You treat your protein with a lower concentration of compound X and you want to know if there is any native (undisrupted) protein left. Which of the following methods would you use? Explain.
(a) dialysis (b) fingerprinting (c) ultracentrifugation (d) native gel electrophoresis (e) SDS-PAGE.

C. Do you think that a compound that disrupts only quaternary structure exists? Are there other structural levels a compound that disrupts quaternary structure ALSO disrupts? Explain.

Reference no: EM132579452

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