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You perform a forward genetic screen to look for Arabidopsis thaliana mutants that are altered in cold stress responses. You find two mutants with opposite phenotypes. Mutant #1 is extremely resistant to cold stress (compared to the wild-type plant), and you identify the gene that has been mutated. It turns out that there is a single base pair mutation that changes a Lysine to an Arginine in the protein. Interestingly, this is the only lysine in this protein which contains 400 amino acids.The other mutant is less tolerant to cold stress than a wild type plant. This mutant contains a different single base pair mutation in the same gene as mutant #1. This mutation changes a codon encoding Glycine that is 10 amino acids from the Nterminal end of the protein, to a Stop codon.Explain why these specific point mutations in the same gene can cause radically different mutant phenotypes. In your answer, please discuss the role this gene plays in the cold response.
A study is planned to compare two weight loss programs in patients who are obese. The 1st program is based on restricted caloric intake and the second is based on specific food combinations.
The baroreceptors are responsible for detecting and responding to changes in mean arterial pressure. Please describe the responses of baroreceptor reflex to both an acute and a chronic increase in mean arterial pressure.
Not much more than 100 years ago, ordinary citizens were unaware of the role of microorganisms in the cause and spread of disease. In her book, "The Gospel of Germs",
A Drosophila experiment analyzing linkage of X-linked genes studied a recessive eye mutation, a recessive wing-vein mutation, and a recessive bristle mutation
Determine the total mass of bovine serum albumin (BSA) in 50 ml of BSA at 4 percent (wt./vol), given in milligrams? What is the answer in micrograms?
Genes A , B , and C are linked on a chromosome and found in the order A-B-C. Genes A and B recombine with a frequency of 10 percent.
If the fox were to feed only on insect-eating birds, how many square meters of plant material would be necessary.
Predict equilibrium potentials E K and E Na at a temperature of 13 o C. Suppose that the Na permeability of the cell membrane is 1/50 that of the K permeability, predict the resting potential, E m , assuming that Cl is in equilibrium.
The child is running a fever, has fflu-like symptoms, and his lymph glands are very swollen and sore to the touch. Of what importance are the swollen and sore lymph glands.
When performing the Kirby-Bauer disk diffusion technique what do the microcolonies in the zone of inhibition represent?
If populations are sustaining growth at r = 0.088 and the population in 2001 is 3000 individuals, in what year would snow geese be taken off an endangered species list.
Explain the action potential in terms of the different functional states of the voltage gated Na+ membrane channels.
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