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Question
You are't asked to pre pare a Master mix using the BGI PCR Kit. 25 samples to process. Since you are only using a half-plate you only need 3 controls. You are only a beginner in reagent preparation and therefore aren't that much confident on yo ur pipetting skills, so you created an additional number for pipetting error which is 2.
Calculate for the:
a. Desired volume for reaction mix
b. Desired volume for enzyme mix
c. Master mix volume
Discuss the process of your reagent preparation on Question 1.
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