Discuss the basic method used to carryout pcr

Questions:

1) Discuss the basic materials needed to be carry out PCR?

2) Discuss the basic method used to carryout PCR?

3) What are the major consideration when designing a PCR primer?

4) Describe why the paramater is important?

5) Describe Q-PCR(quantative )

6) Describe RT-PCR (reserve transcription)

Describe the renin-angiotensin-aldosterone pathway in body : Beta-blockers are another category of drug used to treat hypertension. Summarize the mechanism of beta-blockers.

Differences between bleeding time and clotting time : What are the differences between bleeding time and clotting time?

What is the expected band in gel electrophoresis : What is the two reason cause the difference in observed and expected band in gel electrophoresis. Explain it clearly.

Compare hormone profile of progesterone-estradiol in rate : Compare the hormone profile of progesterone and estradiol in rats and humans during pregnancy, relative to the timing of parturition.

Discuss the basic method used to carryout pcr : Discuss the basic materials needed to be carry out PCR? Discuss the basic method used to carryout PCR?

Examples of the environment affecting the phenotype : Pick one of these examples of the environment affecting the phenotype, and argue if this phenotype is being genetically assimilated.

What are operations involved in beef cattle production : What are the different operations involved in beef cattle production? Describe the different stages of growth and what occurs at each stage.

What structures conduct air vibration to the hair cells : For us to hear sound, what structures conduct air vibration to the hair cells? In what order?

Why should the ethanol-lysate boundary not be disturbed : Why should the ethanol/lysate boundary not be disturbed? Do you think we can use a blender to break open the cells to isolate the DNA?