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BIOL2173 Microbiology - RMIT University
Assessment Questions
Question 1. A mutant you made in the laboratory with 5-bromouracil suddenly regains the wild-type phenotype. You discuss this phenomenon with your advisor and colleagues, and they suggest you try transposon mutagenesis to avoid this problem and create stable mutants. Why is it important to create microbial mutants in research? i.e. whatare mutants from wild type used for? Explain the procedure to create mutants in bacteria, AND discuss why would mutants created with 5-bromouracil be more likely to regain the wild-type phenotype than mutants created via transposon mutagenesis?
Question 2. Antibiotic resistance is a major healthcare problem, with some microbes developing resistance to most known antibiotics. How can microbes develop resistance against currently used antibiotics? Discuss two potential mechanisms for developing antibiotic resistance AND identify potential approaches to overcome it? Support your answer with examples and cited references.
Question 3. Some residential human oral bacteria may cause opportunistic infections in other body systems. Discuss the potential of residential microbes to develop into opportunistic pathogens, why? Where? When? And give an example on an oral Gram-negative bacterium that has the potential to cause opportunistic infectionsin patientswho are diagnosed with inflammatory Bowel Diseases,supporting your answer with cited evidence from the literature.
It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.
This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.
Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.
This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.
Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?
Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.
Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.
The use of PCR and genetic approaches in biotechnology
Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.
What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?
Prepare an essay on nosocomial infection.
To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.
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