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lex A is an E. coli gene whose product regulates the transcription of certain target genes that are located at different positions in the bacterial chromosome. Identified target genes appear to be involved in DNA repair and recombination. Under normal conditions, the LexA protein binds near and represses transcription of target genes; however, when the cell experiences significant DNA damage caused by environmental mutagens, the LexA protein is inactivated and transcription of the target genes occurs. Your research objective is to identify all of the target genes repressed by the LexA protein. (Assume that you have access to a DNA microarray consisting of sequences representing each of the genes in the E. coli genome.) As the starting point for your work, you isolate a lexA mutant strain that produces no functional LexA protein. Briefly describe an experiment that uses a functional genomics approach to identify all of the LexA target genes. Give the basic steps of the experiment and show how it would allow you to achieve your objective.
If succinate is used as the electron donor in oxidative phosphorylation, how many ATPs should be generated for each pair of electrons donated by succinate. explain What does UNCOUPLING mean.
Her breath smells like acetone and her blood glucose test out at 650mg/100ml of blood. She is in acidosis. What hormone must be administered to her and why. Where may she find such DNA.
What is involved in going from genomic DNA to messenger RNA (mRNA)? The transciption process involves the concerted action of several proteins called transcription factors which method the DNA in a multi-step process into mRNA.
What is basics of epidemiology, brief description on Understanding basics of epidemiology
You have been injected into the femoral vein? What is the path from the right femoral vein to the lower lobe of the right lung? What is the path that I would take from the right femorla vein to the lower lobe of the right lung via right pulmonary art..
What are genotypes of the adult that were crossed. 0 percent of a population died from sunburn, of the 10 percent who died 10 individuals were albino and the rest were split evenly between heterozygous (Gg) and dominant individuals (GG). So my questi..
Trace a route of air from the nares to an alveolus. Name subdivisions of organs where applicable, and discriminate between conducting and respiratory zone structures. how large a force does she exert on the sled.
On the basis of these results, advance a hypothesis about the cause of the phenotype. Based on your hypothesis, what do you predict would happen to developing T cell in an HD mouse which has been made transgenic for a TCR restricted to I- A b .
Explain three ways that the knowledge of metabolic processes photosynthesis and cellular respiration can improve your level of physical fitness.
What is the possibility that their next child will show both defects? Demonstrate the work.
Determine which of the following best explains why cardiac muscle is incapable of tetanus, a sustained contraction characteristic of skeletal muscle?
If a transferrin receptor has a cross-sectional area of 50 Angstroms X 100 angstroms (5x10nm), and how many receptors could be accomodated in a 100nm clathrin-coated vesicle assume that only 50% of surface area can be occupied by proteins.
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