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Discuss 2 laboratory techniques to isolate microbes

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1. Compare and contrast the 4 categories of organisms based on their energy and carbon sources.

2. Define and differentiate between anaerobes, aerobes, aerotolerant anaerobes, facultative anaerobes, and microaerophiles.

3. Describe the toxic forms of oxygen and explain why each is toxic. Discuss ways that cells protect themselves from these harmful effects.

4. Discuss the effects of extreme temperature, pH, and osmotic and hydrostatic pressure on microbial growth.

5. Define quorum sensing and discuss the role it plays in biofilm formation.

6. Discuss 2 laboratory techniques to isolate microbes.

7. Define, compare, and contrast the following types of media: defined, complex, selective, differential, enrichment, and anaerobic media.

8. Compare refrigeration, deep freezing, and lyophilization as methods for storing bacterial cultures.

9. Explain the process of binary fission in bacteria.

10. Define generation time and explain how to calculate it.

11. Draw, label, and explain the stages of a typical growth curve for bacteria.

12. Discuss direct and indirect methods for assessing bacterial growth, including pros and cons for each.

Reference no: EM133248426

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