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1)In what order will the following proteins be eluted from anSP-sepharose column by an increasing salt gradient at pH 7: hemoglobin, serum albumin, lysozyme, pepsin, and fibrinogen?
2) In what order will the following proteins be eluted from aG-50 column: cytochrome c, myoglobin, pyruvate decarboxylase, hemoglobin, RNA polymerase, and titin ? Given your answer, whatmight be a more appropriate resin to use to separate this collection of proteins, and what would the elution order be with this more appropriate resin?
Describe how Dendritic cells and T regulatory cells affect immune tolerance. And what woulld happen if there is too much immuno tolerance ?
Explain the common consequences of urinary tract obstructions and describe the etiology, pathophysiology, and unique manifestations of kidney stones, neurogenic bladder, overactive bladder syndrome, and anatomic obstructions to urine flow.
How do protists transport wastes and ions.
You have 0.05 mL of an undiluted culture at a concentration of 3.6 x 106 CFU/ml. You then add 4.95 mL sterile diluent. what is the dilution, and what is the final concentration of the cells?
Agarose gel electrophoresis was conducted on undifferentiated myogenin, differentiated myogenin, undifferentiated GAPDH, differentiated GAPDH, and a negative control ladder. The UV image of the products produced only the ladder. What could cause t..
The cryptic splice site adds two nucleotides to the finished transcript if the cryptic site is used instead of the major splice site. How might this compensate for a deletion that causes a frameshift?
One subunit of tropomyosin is homologous to calmodulin. What would you predict the role of that protein would be?
DNA template sequence of a gene encoding mRNA in E.coli determine the total charge of the protein?
There are 2 heterzygotes: one is wild type chromosome/a chromosome with a deletion, the other one is wild type chromosome/a chromosome with inverted sequence. Which one has higher chance to produce a normal gamete after meiosis? Why?
Eight year old flown into emergency center from rural hospital. He was helpping his father out on a farm. The sketchy history obtained was that the child developed nausea and vomiting.
Based on your knowledge of the role of renal tubule in regulating ion balance, explain how a diuretic could induce hyperkalemia. Then explain how a different diuretic might induce hypokalemia.
What happens to a virus that uses the rolling circle model of replication but has no means to cut monomers at discrete sites and cuts only unit lengths?
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