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Discover a cool new "type II" restriction enzyme that cuts DNA whenever it finds the sequence: 5'-TCCRAC(N)20-3' , where "R" means the base could be an "A" or a "G", and "(N)20" means any sequence of 20 nucleotides long. In theory, how many cuts per kilobase of DNA would this enzyme make in the human genome, assuming that the human genome is 3 x 109 bp long and is 40% GC?
The wild type allele of a bacterial gene is easily selected by growth on a special medium, if the enzyme HindIII is used, then clones are easily found
Explain three ways that the knowledge of metabolic processes photosynthesis and cellular respiration can improve your level of physical fitness.
The normal sequence of aparticular protein is given here, along with several mutantversions of it. For each mutant, explain what mutation occurred in the coding sequence of the gene. Assume only a single mutationalevent explains each altered amino..
What is competent cell? Why can a competent cell take up plasmid DNA? What is a method that some scientists use to make competent cells and briefly describe it.
Although several different mammalian species have been cloned, the efficiency of this process is extremely low. Often tens or even hundreds of oocytes must be implanted with donor nuclei to obtain one healthy live birth.
What catalyzes the formation of the peptide bond during traslation?
Trypsin and elastase are both enzymes that catalyze hydrolysis of peptide bonds. But trypsin only cuts next to lysine, and elastase only cuts next to alanine.Why?
You are a geneticist who has a single copy of a gene that you wish to sequence. However, DNA sequencing requires multiple copies of the gene
During mitosis it is important for sister chromatids to stick together (called "cohesion") during some stages and then separate. Researchers have identified proteins called "cohesins" that are involved in sister chromatid cohesion. If you wanted t..
Using sickle-cell anemia as a basis, describe what is meant by amolecular or genetic disease. What are the similarities anddissimilarities between this type of disorder and a disease causedby an invading microorganism?
If you have 10 different amino acids and want to make a polypeptide chain of 6 amino acids, how many theoretical combinations of amino acids could you make?
Write a 700- to 1050-word paper on a recent disaster (e.g. Exxon Valdez oil spill, Hurricane Katrina) and address the following: •Give a brief overview of the impacted ecosystem including a description of the wildlife and human populations. •Give an ..
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